Biotin's positive effects in treating hoof abnormalities in horses led to speculation on the nutrient's potential benefit in humans with brittle nails. Given the biochemical similarities between horse hooves and human fingernails and toenails, this speculation was not unreasonable, and a number of preliminary clinical studies suggest that biotin does strengthen nails.5, 29
Additionally, both vitamin B6 and biotin help produce keratin - the predominant protein in nails. Biotin is also involved in the production of collagen and elastin, proteins for healthy skin structure beneath the nail.19
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